Analysis Note

Appearance (clearness): clearAppearance (color): yellowish-brownpH-value (25 °C): 7.2 - 7.6Growth promotion test in accordance with the current version of DIN EN ISO 11133.Mixed inoculum of E.coli and S.aureusAdditionaly monoculture of S.aureusInoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)): ≤ 100Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 10000Growth (Escherichia coli ATCC 35150 (O157)): >10 yellowish-brown colonies on CT-SMAC agarGrowth (Escherichia coli ATCC 700728 (WDCM 00014)): >10 yellowish-brown colonies on CT-SMAC agarGrowth Escherichia coliATCC 25922 (WDCM 00013): >10 pink colonies on CT-SMAC agarGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): total inhibition on TSAIncubation:mTSB broth: 18 - 24 hours at 41,5 °C, aerobicConfirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic

Application

mTSB broth with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli (EHEC) as well as detection of E. coli serotype O157:H7 in foods.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Modified Tryptic Soy Broth with Novobiocin is recommended for the enrichment of enterohemorrhagic E. coli (EHEC). The nutrients in mTSB broth provide favorable growth conditions, while the mixture of bile salts No. 3 and Novobiocin suppress the growth of the Gram-positive microbial flora.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 g in Plastic bottle